In 2015 (October-December) a detailed documentation was performed in order to achieve the specified objectives. A standardized questionnaire and a consent form have been developed. Additionally, work protocols were updated and a booklet was created for informing the survey participants (see caption Other results).
During 2016 (January to December) the participants selection for the study was done: 40 women in the target area (Băiţa, Nucet, Fînaţe, Cîmpani) and 40 women in the control area (Cociuba Mică, Gurani, Pietroasa). Glass retrospective dosimetry detectors were placed, along with radon, thoron and decay products detectors for contemporary evaluation and the local water supply was sampled.
During this stage, the work protocol was improved for the film developing of LR-115 detectors used in retrospective dosimetry of Po-210 withheld in household glass. An installation containing acrylic slides was created for storing 3 detectors of 3x3 cm and a PTFE (polytetrefluoroethylen) carousel for inserting 12 slides, respectively. Thus, the system enables the simultaneous developing of 36 film detectors with the specified conditions mentioned in the scientific literature. The setup is self developed and guided only by the specifications mentioned in articles in the corresponding field. The retrospective evaluation of the radon activity concentration of the two studied zones was performed through the film detectors developing. Also, through measurements of radon and radium activity in water samples, the annual effective dose by ingestion was calculated.
During the months of August and September, blood samples were taken from 76 of the 80 patients in Family medicine cabinets. The collected blood was transported, separated and stored in the Radiobiology laboratory at the Institute of Oncology "Prof. dr. Ion Chiricuţă". The first assessment was performed for establishing the degree of DNA damage through two tests (comet and micronuclei), as well as a benchmarking of the repair kinetics.
In 2017, the second campaign of measuring indoor radon and thoron concentrations was completed. After the etching of the detectors, the annual effective dose was assess based on the measurements made (indoor radon and thoron concentrations, radon and thoron progenies concentrations, gamma dose, radon and radium in water samples). At the same time, the two cytogenetic tests were applied in order to assess the degree of damage induced to the DNA. The degree of dependence between the identified DNA lesions and the annual effective dose was evaluated. The obtained results are described in detail in Report 2017.
Part of the results were presented at the "8th International Conference on Protection against radon at home and at work, 12-16 September 2016 Prague, Czech Republic", and Third East-European Radon Symposium, 15-19 May 2017, Sofia, Bulgaria, respectively. Two ISI publications were published and another one is under review.
As a derived result of the present project, two international collaboration agreements were established (prof. Dr. D. Pressyanov - University of Sofia, Bulgaria and Dr. Rosaline Mishra - Bhabha Atomic Research Centre, Mubai, India, respectively).
1. Dolha Monica, Timar-Gabor Alida, Dicu Tiberius, Cosma Constantin (2017) Measurements of terrestrial gamma dose rates and radon concentrations from indoor air and water in Transylvania region, Romanian Reports in Physics 69 Click here
2. Cucoş A., Papp B., Dicu T., Moldovan M., Burghele B., Moraru I., Tenţer A., Cosma C., (2017) Residential, soil and water surveys in north-western part of Romania, Journal of Environmental Radioactivity 166, 412 - 416 http://dx.doi.org/10.1016/j.jenvrad.2016.10.003 Click here
3. Dicu T., Burghele B., Lupulescu D., Cucoş A., (2018) The challenge in using the retrospective assessment of residential radon concentration, Radiation Protection Dosimetry 181, 20 - 25 Click here
4. Dicu T., Burghele B., Cucoş A., Mishra R., Sapra B. K., Assessment of annual effective dose from exposure to natural radioactivity sources in a case – control study in Bihor County, Romania, Radiation Protection Dosimetry, in press
Title: Assessment of DNA damage related to chronic low-dose ionizing radiation exposure in population from Băița-Ştei area
Authors: Dicu Tiberius, Virag Piroska, Cucoş Alexandra, Burghele Bety, Paşcu Andrada, Dolha Monica
Conference: 8th International Conference on Protection against radon at home and at work, 12 – 16 September 2016, Prague, Czech Republic
Title: Comparison of retrospective and contemporary radon measurements in dwellings in Romania
Authors: Burghele Bety, Dicu Tiberius, Cucoş Alexandra
Conference: Third East-European Radon Symposium, 15-19 May 2017, Sofia, Bulgaria (TEERAS 2017)
The installation containing acrylic slides was created for storing 3 LR-115 detectors of 3x3 cm and a PTFE (polytetrefluoroethylen) carousel for inserting 12 slides, respectively.
- two international collaboration agreements were established (prof. Dr. D. Pressyanov - University of Sofia, Bulgaria and Dr. Rosaline Mishra - Bhabha Atomic Research Centre, Mubai, India, respectively).
- Brochure - Information for participants
The expected results will enable to create a database of genotoxic effects, from exposure to low doses of ionizing radiation, for residents living near the closed Băița uranium mine. For this purpose, 40 people from the target area will take part in the study (Băița-Ștei), along with 40 people from an area with a normal radioactive background. The participants in the study will be required to sign a written consent of acceptance regarding the purpose of the study, the type of analysis performed and the publication of results, ensuring a confidential collaboration. The consent form along with a general health questionnaire and information related to the specific household and building materials will be collected for this project. In order to achieve the objective, the detectors described under Objective 1 will be placed for each participant, and a sample of blood will be taken for testing via the comet assay and micronucleus test. The evaluation of the repair kinetics will be performed by exposure to a 2 Gy dose of gamma radiation. Participants in the study will receive regular newsletters on the radon and thoron results measured in their dwellings analysis and the results from the comet test and micronucleus tests in lymphocytes, respectively.